Necrosis and graft incompatibility

Graft incompatibility is often due to failed vascular connection in the graft junction. Necrosis in the junction is a common symptom of a failed vascular junction. This work focuses on quantifying the degree of necrosis inside the junction of incompatible grafts with DAB staining and Instron tensile force machines. Skills: Grafting, plant care DAB stainingContinue reading “Necrosis and graft incompatibility”

Intercellular communication within the graft junction

During the healing process following grafting, meristematic tissue must initiate xylem and phloem cells within the graft junction. Additionally, the files of the scion (upper portion of the graft) and stock (lower portion of the graft) must coordinate their placement, so that new vasculature can connect the two portions. In order for this coordinated healingContinue reading “Intercellular communication within the graft junction”

Anatomy and genetics of graft compatibility

Anatomical features from the graft junctions of tomato:tomato, pepper:pepper, tomato:pepper, and pepper:tomato over time were used to inform bioinformatic analysis. Skills: Grafting, plant care Histology and confocal microscopy (propidium iodide, auramine O, calcufluor white) Bioinformatics (RNA seq analysis) Statistics See associated publication: Gene regulatory networks for compatible versus incompatible grafts identify a role for SlWOX4Continue reading “Anatomy and genetics of graft compatibility”

Vegetable Grafting

Grafting is an ancient technique where separate plants are combined into a single individual. For thousands of years humans have used grafting as a means of propagation, yet there is still much unknown about the process. Although most commercial grafting occurs in woody crops, more and more herbaceous plants are being grafted. The New YorkContinue reading “Vegetable Grafting”